Service introduction
In recent years, the research on the genomes of pathogenic microorganisms has made rapid progress. Genome research refers to the sequencing of the entire genome of microorganisms, and on the basis of understanding the overall gene structure, the functions of each gene alone or the interaction between several genes are studied. Through genome research, the complete genes of microorganisms are fundamentally revealed, which can not only discover new interactions between genes, new regulatory factors, etc., but also enable humans to master the pathogenic mechanism and laws of pathogenic microorganisms at a higher level. This will lead to the development of new preparations, vaccines and drugs for the diagnosis, prevention and treatment of microbial infections.
According to different research purposes, microbial genome sequencing is mainly divided into four types: microbial genome survey, microbial genome framework, fine map and finished map. Our company uses the Illumina platform for bacterial genome sequencing, using short-fragment high-coverage sequencing, combined with Illumina paired-end sequencing, to provide personalized solutions for different sequencing studies.
Assembly evaluation standards
Service type | Completion standard |
Microbial genome survey | Sequencing depth reaches 50X |
Microbial genome framework | Genome coverage is greater than 95%, gene region coverage is greater than 98% |
Microbial genome fine map | Genome coverage is greater than 98%, gene region coverage is greater than 99% |
Technical procedures
Bioinformatics analysis
生物信息學(xué)分析 | |||
基因組調(diào)查信息分析 | 基因組精細(xì)圖信息分析 | 基因組框架圖信息分析 | 基因組完成圖信息分析 |
原始數(shù)據(jù)統(tǒng)計(jì) | 原始數(shù)據(jù)統(tǒng)計(jì) | 原始數(shù)據(jù)統(tǒng)計(jì) | 原始數(shù)據(jù)統(tǒng)計(jì) |
序列的初步組裝 | 序列組裝 | 序列組裝 | 序列組裝 |
基因組大小的預(yù)測(cè) | ORF 預(yù)測(cè)和基本功能注釋 | ORF 預(yù)測(cè)和基本功能注釋 | ORF 預(yù)測(cè)和基本功能注釋 |
GC 含量和重復(fù)序列的預(yù)測(cè) | 完成圖繪制的建議 | 精細(xì)圖繪制的建議 | 比較基因組學(xué)和功能基因組學(xué)的分析 |
Sample requirements
實(shí)驗(yàn)類型 | RNA樣品總量 | 樣品濃度和純度 | 樣品保存 | 樣品選擇 |
微生物基因組denovo測(cè)序 | 小片段文庫(kù)樣品制備需要總量 3 μg 的樣品,mate-pair 文庫(kù)(2 kb、5 kb、10 kb)樣品制備需要 10-50 μg 的樣 | 樣品濃度 >100 ng/μl,OD260/280 介于 1.8-2.0 之間,無(wú)肉眼可見(jiàn)污染 | 請(qǐng)選擇干粉、酒精、TE 或超純水的保存方式之一。 | 對(duì)于細(xì)菌和真菌樣品,希望盡量來(lái)自于同一個(gè)菌株,不要有宿主 DNA 污染 |